ApaCl, an isoschizomer of BamHl isolated fromAcetobacter pasteurianus
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چکیده
منابع مشابه
Isolation and purification of CeqI endonuclease, an isoschizomer of EcoRV.
Ooryrprvcterium equii produces a new restriction endonuclease, an iso-schizemer of EcoKV (1). The bacterial strain does not contain significant levels of other nucleases, so the restriction endonuclease activity can be detected in crude extracts. Ihe enzyme is purified by ammonium sulphate fractionation (50-70 % saturation) and DEAE cellulose chranatography (elutes with 200 mM KC1 in PC buffer)...
متن کاملIsolation and identification of the restriction endonuclease PtaI from Phormidium tadzschicicum, an isoschizomer of BspMII.
Ptal is a type II restriction endonuclease from the Cyanobacterial strain Phormidium tadzschicicum. This strain was obtained from the Collection of Algal Cultures of Leningrad University. Ptal recognizes the sequence 5'-TCCGGA-3' and cleaves between T and C. The enzyme was purified using the following chromatographic steps: 1) Phosphocelulose, 2) DEAESephadex. The enzyme was free of contaminati...
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in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...
15 صفحه اولAsymmetric DNA recognition by the OkrAI endonuclease, an isoschizomer of BamHI
Restriction enzymes share little or no sequence homology with the exception of isoschizomers, or enzymes that recognize and cleave the same DNA sequence. We present here the structure of a BamHI isoschizomer, OkrAI, bound to the same DNA sequence (TATGGATCCATA) as that cocrystallized with BamHI. We show that OkrAI is a more minimal version of BamHI, lacking not only the N- and C-terminal helice...
متن کاملA restriction endonuclease (DpaI) from Deleya pacifica IAM 14115, a marine bacterium, an isoschizomer of ScaI.
physicochemical properties. Deleya pacifica (Baumann et al. 1972) Baumann et al. 1983 IAM 141 15*1 was aerobically grown for 24hr at 30°C in a modified marine broth as described previously.2) The cells (30g) harvested from 6liters of culture broth by centrifugation were suspended in 200ml of a sonication buffer (pH 7.5) containing 10mM K2HPO4, 7mM 2-mercaptoethanol, 100mM NaCl and 20/xg/ml phen...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.13.3513